Fastidious anaerobe agar compared with Wilkins-Chalgren agar, brain heart infusion agar, and brucella agar for susceptibility testing of Fusobacterium species

Detection with Agar Dilution Susceptibility Testing

A dye reduction method for determining a definitive endpoint with agar dilution susceptibility testing has been developed. Bacterial growth was determined by applying to the inoculum spot a dye solution containing 2-(p-iodophenyl)-3-(pnitrophenyl)-5-phenyltetrazolium chloride and phenazine methosulfate. Viable colonies reduced the tetrazolium salt to a visible red color within 3 to 5 min. The m...

Comparison of Supplemented Brucella Agar and Modified Clostridium difficile Agar for Antimicrobial Susceptibility Testing of Clostridium difficile

BACKGROUND Antimicrobial susceptibility testing (AST) of Clostridium difficile is increasingly important because of the rise in resistant strains. The standard medium for the AST of C. difficile is supplemented Brucella agar (sBA), but we found that the growth of C. difficile on sBA was not optimal. Because active growth is critical for reliable AST, we developed a new, modified C. difficile (m...

Agar Gradient Diffusion Susceptibility Testing for Neisseria gonorrhoeae: A Reliable Alternative to Agar Dilution?

The Introduction of Agar-agar into Bacteriology.

Among fundamental developments in bacteriology the elaboration of solid culture substrates will always hold first place. With the introduction of a method for isolating micro6rganisms and growing them in pure culture, bacteriologists possessed the means for studying what Ferdinand Cohn (1872) called "Die kleinsten lebenden Wesen." Credit for the discovery of the solid medium technique is common...

Blood agar, chocolate agar, and Mycobacterium tuberculosis.

We have read with interest the recent paper by Drancourt et al. in which they reviewed the use of blood agar medium for the primary isolation of Mycobacterium tuberculosis (2). They state that to their knowledge “no comparative study comparing the efficacy of blood-based agars and egg-based agars has been carried out, and even the ability of blood agar to support growth of M. tuberculosis was f...